|
KMID : 0942820060050010029
|
|
Journal of Korean Brain Tumor Society
2006 Volume.5 No. 1 p.29 ~ p.38
|
|
|
Activation of Rat Marrow Stromal Cells into Immune Effector Cells
|
|
Kim Hyo-Chang
Kang Seok-Gu
Jeun Sin-Soo
Yoo Do-Sung
Huh Pil-Woo
Cho Kyoung-Seok
Kim Dal-Soo
Park Chun-Kun
|
|
|
Abstract
|
|
|
Objective: Marrow stromal cells(MSCs) have been shown to have the capacity of orthodox and unorthodox plasticity. In this study, the authors tried to differentiate MSCs into immune effector cell and also to access in vitro cytotoxicity of MSCs from rat.
Methods: Rat MSCs(rMSCs) were isolated by standard methodology and were activated by interleukin-2(IL-2), interleukin- 15(IL-15), colony stimulating factor GM-CSF, and combinations. Phenotypes of these various activated cells were determined using flow cytometry. The secreted protein from effector cells was estimated by enzyme-linked immunosorbent assay. Cytotoxicity of rMSCs and activated rMSCs against the target cells(9L rat glioma cell line) were estimated using visual survival cell assay. The expression of immune response related genes in activated cells was measured.
Results: After various cytokines activation of rMSCs, the population of immune effector cells(CD8, CD161a) and immune reaction related proteins(IL-4, γ-INF) were increased. There was a significant cytotoxicity of rMSCs activated with various cytokine combinations. Apoptosis may be one of the lysis mechanism of target cells by activated rMSCs. The contributing genes could be γ-INF, FasL and perforin.
Conclusion: This study suggests that rMSC may differentiate into immune effector cells and have cytotoxic capability against malignant glioma cells. However, we have to investigate orthotopic animal study for the proper translation.
|
|
|
KEYWORD
|
|
|
Cytokine, Cytotoxicity, Malignant glioma, Marrow stromal cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|